Bacteriophage recombineering in the lytic state using the lambda red recombinases
نویسندگان
چکیده
منابع مشابه
Bacteriophage recombineering in the lytic state using the lambda red recombinases
Bacteriophages, the historic model organisms facilitating the initiation of molecular biology, are still important candidates of numerous useful or promising biotechnological applications. Development of generally applicable, simple and rapid techniques for their genetic engineering is therefore a validated goal. In this article, we report the use of bacteriophage recombineering with electropor...
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The impact of phage lambda: from restriction to recombineering.
Experiments using phage lambda provided early insights into important molecular mechanisms, including genetic recombination and the control of gene expression. Before recombinant DNA technology, the use of lambda, most particularly lambda transducing phages, illustrated the importance of cloning bacterial genes, already providing some insight into how to use cloned genes to advantage. Subsequen...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2011
ISSN: 1751-7915
DOI: 10.1111/j.1751-7915.2011.00292.x